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1.
Microbiology ; (12)2008.
Article in Chinese | WPRIM | ID: wpr-686442

ABSTRACT

At the present time, training student to have innovative and applied ability has become the object of higher education in China. In this paper, it was proposed that teacher was obligated to create certain atmospheres in classroom to achieve this goal, including friendliness, harmoniousness, encouragement, happiness, discussion, exploration, etc. At the same time, student-centered study should be encouraged. Through these measures, the spirit of innovation will be inspired, the applied ability will be trained, the capability of self-study will be enhanced.

2.
National Journal of Andrology ; (12): 628-631, 2007.
Article in Chinese | WPRIM | ID: wpr-297669

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the distribution and the antimicrobial resistance of the bacteria, mycoplasma and Chlamydia trachomatis isolated from the prostatic secretion of infertile men with chronic prostatitis, and to provide clinicians with grounds for choosing antibiotic agents.</p><p><b>METHODS</b>The bacteria obtained were isolated and identified, the Chlamydia trachomatis was detected by FLO-PCR, and the results were analysed statistically.</p><p><b>RESULTS</b>In 1 186 specimens of EPS, the total positive rate of isolates was 51.7%. Among them, there were 364 strains of gram-positive coccus, 20 gram-negative bacillus, 5 other strains and 157 mycoplasma, including 116 Ureaplasma urealyticum and 41 Mycoplasma hominis, and 67 Chlamydia trachomatis DNA. As for the isolated staphylococci, their antimicrobial resistance was the lowest against vancomycin (0.0%), but the highest against penicillins (76.9%-100%); for the Streptococcus agalactiae, it was the highest against erythromycin and clindamycin (100%), and the lowest against beta-lactams, aminoglycosides, trimethoprim + sulfamethoxazole, rifampin and vancomycin (0.0%); for the Ureaplasma urealyticum, it was the highest against ciprofloxacin (59.5%), and the lowest against josamycin, tetracycline and fosfomycin (1.7%); for the Mycoplasma hominis, it was the highest against erythromycin (100%), and the lowest against doxycycline and fosfomycin (0.0%).</p><p><b>CONCLUSION</b>Bacteria, mycoplasma and Chlamydia trachomatis are the possible etiological factors of male infertility. Isolated bacterial strains differ greatly in their resistance against different antibiotics.</p>


Subject(s)
Adult , Humans , Male , Middle Aged , Anti-Bacterial Agents , Pharmacology , Chlamydia trachomatis , Drug Resistance, Bacterial , Infertility, Male , Microbiology , Microbial Sensitivity Tests , Mycoplasma hominis , Prostate , Microbiology , Pathology , Bodily Secretions , Prostatitis , Microbiology , Ureaplasma urealyticum
3.
Chinese Journal of Medical Instrumentation ; (6): 88-90, 2007.
Article in Chinese | WPRIM | ID: wpr-323304

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the feasibility of tracheal reconstruction with a prosthesis made of memory alloy coated with collagen sponge in mongrel dogs.</p><p><b>METHOD</b>The basic skeleton of the prosthesis was knitted with Ni-Ti memory alloy wires. The tubular mesh was sealed with polyurethane membrane and then inner and external walls of the lumen were coated with collagen sponge. Cervical trachea segmental reconstruction was performed in 8 mongrel dogs with these prostheses. The efficacy of the implanted prostheses were periodically evaluated after operation using x-ray, tracheoscopy and specimen microscope examinations.</p><p><b>RESULTS</b>One dog died of prosthesis dislocation 10 days after operation, another was killed 45 days later because of anastomotic stenosis. 6 dogs survived more than 90 days and the longest one lived for 150 days. Its implanted prosthesis was completely incorporated with the recipient tissue, where re-epithelialization occluded on anastomotic sites. The tracheal lumen was patent.</p><p><b>CONCLUSION</b>This memory alloy tracheal prosthesis has been proved useful for reconstruction of large, circumferential tracheal defects, although its long-term safety and efficiency need to be confirmed.</p>


Subject(s)
Animals , Dogs , Alloys , Artificial Organs , Collagen , Prosthesis Design , Trachea
4.
Chinese Journal of Medical Instrumentation ; (6): 196-198, 2006.
Article in Chinese | WPRIM | ID: wpr-355416

ABSTRACT

It is difficult to perform a primary anastomosis when a pathological trachea is over-lengnthing resected. Thus the tracheal prosthesis is required to apply in the airway reconstruction. Severe defects in the early tracheal prosthesis result in failure of surgery. Great progress has been achieved in recent years as new materials and tissue-engineering techniques are applied. These efforts bring a bright future to clinical applications of tracheal prostheses.


Subject(s)
Animals , Dogs , Humans , Alloys , Anastomosis, Surgical , Biocompatible Materials , Collagen , Prostheses and Implants , Prosthesis Design , Prosthesis Implantation , Methods , Tissue Engineering , Titanium , Trachea , General Surgery
5.
Microbiology ; (12)1992.
Article in Chinese | WPRIM | ID: wpr-684981

ABSTRACT

The research status is reviewed on taxonomic position, ecology, biological active matter and artificial cultivation of Shiraia bambusicola. Following problems are presented: whether there are new species and host specificity of Shiraia bambusicola. Since the artificial cultivation of stroma of Shiraia bambusicola has not been success, Shiraia bambusicola is in situation of emerge of itself and perish of itself so that it is not beneficial to resource protection and continual utilization of this medicinal fungus. It is pointed out that molecular biology of Shiraia bambusicola should be further researched and technology of artificial cultivation of Shiraia bambusicola should be explored in order to exploit and usage of Shiraia bambusicola.

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